In Vitro and In Silico Analysis for Antibacterial Activitiy of Various Extracts of Gracilaria salicornia (Rhodophyta) from Selayar Islands, Indonesia

This study aims to evaluate the toxicity, antibacterial activity, and chemical profiling of Gracilaria salicornia extract. The research stages included extraction, bioactivity test, GC-MS, docking analysis. activity showed better sensitivity against Escherichia coli than Staphylococcus aureus. inhibition zone E. ranged from 7.73-17.30 mm, while S. aureus 6.91-13.44 all extracts were toxic Artemia salina Leach. chloroform extract highest with clear diameter was 17.3±1.65 mm 13.44 ± 0.04 respectively. presence steroids, terpenoids, fatty acids, alkane based on GC-MS analysis responsible for these activities. Molecular that Cholest-5-en-3-ol Cholesta-4,6-dien-3-ol interactions control binding energy -9.99 -9.67 kcal.mol-1 5BMM, -8.00 -7.93 3LPS Based this data, it can be stated G. is highly potential as source compounds.